Quantitative PCR assays for vector copy number and viral detection include testing for VSV-G, GALV, Baboon Endogenous Virus, Cocal Virus, Amphotropic 4070A and RD114 envelope DNA. Investigators may send blood, cells or DNA for analysis.
Replication Competent Lentivirus (RCL) Biologic Assay for virus testing on vector supernatant and end-of-production cells.
Replication Competent Retrovirus (RCR) Extended Biologic Assay for virus testing on vector supernatant and end-of-production cells for RD114 Pseudotyped, GALV Pseudotyped and Other Amphotropic and Xenotropic Viruses.
Direct RCR.
Ecotropic RCR.
Endotoxin Testing (Gel Clot).
PCR for viral vector target sequences: E1A, SV40 and VSV-G
Vector Insert Stability Assay for retroviral and lentviral vectors. Sequencing is performed as required to identify the vector insert.
Insertion Site Testing Analysis by PCR and next-generation sequencing aims to identify the specific site of vector integration within the genome and estimate the frequency of a specific intergration within a population of cells.
Cell Line Identity by ADA Isoenzyme Identification.