Sastry, L., Xu, Y., Johnson, T., Desai, K., Rissing, D., Marsh, J., and Cornetta, K. Certification assays for HIV1-based vectors: Frequent passage of gag sequences without evidence of replication competent viruses. Molecular Therapy 8:830-839, 2003.
Cornetta, K., Yao, J., Jasti, A., Koop, S., Douglas, M., Hsu, D., Couture, L.A., Hawkins, T., Duffy, L. Replication-competent lentivirus analysis of clinical grade vector products. Mol Ther. 19:557-566, 2011. PMCID: PMC3048188
Cornetta, K., Duffy, L., Turtle, C.J., Jensen, M., Forman, S., Binder-Scholl, G. Fry, T., Chew, A., Maloney, D. G., and June, C.H. Absence of Replication Competent Lentivirus in the Clinic: Analysis of Infused T Cell Products. Molecular Therapy 28:280-288. 2018. PMC5762981
Cornetta, K., Koop, S., Nance, E., House, K., Duffy, L. Replication-competent lentivirus analysis of vector-transduced T cell products used in cancer immunotherapy clinical trials. In: Swiech, K., Ribeiro Malmegrim, K. C., Picanco-Castro, V. eds. Methods in Molecular Biology: Chimeric Antigen Receptor T cells. Humana Press, 2020; 2086:181-194
Replication Competent Retrovirus (RCR):
Cornetta, K., Nguyen, N., Morgan, R.A., Muenchau, D.D., Hartley, J., and Anderson, W.F. Infection of human cells with murine amphotropic replication-competent retroviruses. Human Gene Therapy 4, 579-588, 1993.
Chen, J., Reeves, L., and Cornetta, K. Safety testing for replication-competent retrovirus (RCR) associated with gibbon ape leukemia virus pseudotyped retroviral vectors. Human Gene Therapy 12:61-70, 2001.
Reeves, L., Duffy, L., Koop, S., Fyffe, J., Cornetta, K. Detection of ecotropic replication-competent retroviruses: Comparison of S+/L- and marker rescue assays. Human Gene Therapy 13:1783-1790, 2002.
Duffy, L., Koop, S., Fyffe, J., and Cornetta, K. Extended S+/L- assay for detecting replication competent retroviruses pseudotyped with the RD114 viral envelope. Preclinica May/June:53-59, 2003.
Cornetta, K., Duffy, L., Feldman, S.A., , Mackall, C.A., Davila, M.L., Curran, K.J., Junghans, R.P., Tang, J.Y., James Kochenderfer, J., O’Cearbhaill, R., Archer, G., Kiem, H-P., Shah, N.L., Delbrook, C., Kaplan, R., Brentjens, R. J., Riviére, I., Sadelein, M., and Rosenberg, S.A. Screening Clinical Cell Products for Replication Competent Retrovirus: The National Gene Vector Biorepository Experience. Molecular Therapy: Methods & Clinical Development (2018) https://doi/org/10.1016/j.omtm.2018.08.006 PMC6134358
Other Assays:
Sastry, L, Xu, Y., Duffy, L., Koop, S., Jasti, A., Roehl, H., Jolly, D., and Cornetta, K. Product enhanced reverse transcriptase (PERT) assay for replication competent retrovirus and lentivirus detection. Human Gene Therapy 16:1227-1236, 2005.
National Gene Vector Biorepository:
Cornetta, K. and Matheson, L. The National Gene Vector biorepository’s pharm/tox database. Molecular Therapy 17:582-584, 2009. PMCID: PMC2835100
Cornetta, K. , Matheson, L., Long, R., Duffy, L. The National Gene Vector Biorepository. Eleven Years of Providing Resources to the Gene Therapy Community. Human Gene Therapy: 31, 145-150, 2020. PMC7047076
